Enzymatic and genetic characterization of lignin depolymerization by Streptomyces sp. S6 isolated from a tropical environment.

The conversion of lignocellulosic biomass into bioethanol or biochemical merchandise requires an important pretreatment course of to breakdown the recalcitrant lignin construction. This analysis focuses on the isolation and characterization of a lignin-degrading bacterial pressure from a decaying oil palm empty fruit bunch (OPEFB).

The remoted pressure, recognized as Streptomyces sp. S6, grew in a minimal medium with Kraft lignin (KL) as the only carbon supply. A number of identified ligninolytic enzyme assays have been carried out, and lignin peroxidase (LiP), laccase (Lac), dye-decolorizing peroxidase (DyP) and aryl-alcohol oxidase (AAO) actions have been detected. A 55.3% discount within the molecular weight (Mw) of KL was noticed after 7 days of incubation with Streptomyces sp.

S6 primarily based on gel-permeation chromatography (GPC). Gasoline chromatography-mass spectrometry (GC-MS) additionally efficiently highlighted the manufacturing of lignin-derived fragrant compounds, reminiscent of 3-methyl-butanoic acid, guaiacol derivatives, and 4,6-dimethyl-dodecane, after therapy of KL with pressure S6. Lastly, draft genome evaluation of Streptomyces sp.

S6 additionally revealed the presence of robust lignin degradation equipment and recognized numerous candidate genes chargeable for lignin depolymerization, in addition to for the mineralization of the decrease molecular weight compounds, confirming the lignin degradation functionality of the bacterial pressure.

Self-Healing Dyes – Keeping The Promise?

Self-healing dyes have emerged as a brand new promising class of fluorescent labels. They include two items, a fluorescent dye and a photostabilizer. The latter heals at any time when the fluorescent dye is endangered of taking a response pathway in direction of photobleaching.

EIF3C Antibody

47321-100ul 100ul
EUR 302.4

EIF3C siRNA

20-abx901680
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

EIF3C siRNA

20-abx915165
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

EIF3C siRNA

20-abx915166
  • EUR 661.20
  • EUR 878.40
  • 15 nmol
  • 30 nmol

EIF3C Antibody

1-CSB-PA857867LA01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3C. Recognizes EIF3C from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200

EIF3C Antibody

1-CSB-PA007531GA01HU
  • EUR 716.40
  • EUR 399.60
  • 150ul
  • 50ul
Description: A polyclonal antibody against EIF3C. Recognizes EIF3C from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

EIF3C Rabbit pAb

A7022-100ul 100 ul
EUR 369.6

EIF3C Rabbit pAb

A7022-200ul 200 ul
EUR 550.8

EIF3C Rabbit pAb

A7022-20ul 20 ul
EUR 219.6

EIF3C Rabbit pAb

A7022-50ul 50 ul
EUR 267.6

EIF3C Conjugated Antibody

C47321 100ul
EUR 476.4

EIF3C cloning plasmid

CSB-CL857867HU-10ug 10ug
EUR 669.6
Description: A cloning plasmid for the EIF3C gene.

Anti-EIF3C antibody

PAab02703 100 ug
EUR 463.2

anti- EIF3C antibody

FNab02703 100µg
EUR 658.5
Description: Antibody raised against EIF3C

Anti-EIF3C antibody

STJ29102 100 µl
EUR 332.4

pCMV-SPORT6-EIF3C

PVT14144 2 ug
EUR 594

EIF3C Polyclonal Antibody

A72797-050 50 ul
EUR 302.5

EIF3C Polyclonal Antibody

A72797-100 100 ul
EUR 423.5

EIF3C Polyclonal Antibody

A67130-020 20 ul
EUR 117.7

EIF3C Polyclonal Antibody

A67130-050 50 ul
EUR 302.5

EIF3C Polyclonal Antibody

A67130-100 100 ul
EUR 423.5

EIF3C Polyclonal Antibody

A67130
  • EUR 684.66
  • EUR 117.70
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

EIF3C Polyclonal Antibody

A72797
  • EUR 302.50
  • EUR 423.50
  • 50 ul
  • 100 ul

EIF3C Antibody, HRP conjugated

1-CSB-PA857867LB01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3C. Recognizes EIF3C from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

EIF3C Antibody, FITC conjugated

1-CSB-PA857867LC01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3C. Recognizes EIF3C from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

EIF3C Antibody, Biotin conjugated

1-CSB-PA857867LD01HU
  • EUR 380.40
  • EUR 402.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3C. Recognizes EIF3C from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

Human EIF3C shRNA Plasmid

20-abx955698
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Mouse EIF3C shRNA Plasmid

20-abx974708
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

Rat EIF3C shRNA Plasmid

20-abx988626
  • EUR 961.20
  • EUR 1345.20
  • 150 µg
  • 300 µg

EIF3C ELISA KIT|Human

EF009339 96 Tests
EUR 826.8

Bovine EIF3C ELISA KIT

ELI-26098b 96 Tests
EUR 1113.6

Mouse Eif3c ELISA KIT

ELI-09164m 96 Tests
EUR 1038

Human EIF3C ELISA KIT

ELI-09999h 96 Tests
EUR 988.8

Eif3c ORF Vector (Rat) (pORF)

ORF066450 1.0 ug DNA
EUR 607.2

EIF3C ORF Vector (Human) (pORF)

ORF003466 1.0 ug DNA
EUR 114

Eif3c ORF Vector (Mouse) (pORF)

ORF043759 1.0 ug DNA
EUR 607.2

EIF3C Polyclonal Antibody, HRP Conjugated

A67131-050 50 ul
EUR 302.5

EIF3C Polyclonal Antibody, HRP Conjugated

A67131-100 100 ul
EUR 423.5

EIF3C Polyclonal Antibody, FITC Conjugated

A67132-050 50 ul
EUR 302.5

EIF3C Polyclonal Antibody, FITC Conjugated

A67132-100 100 ul
EUR 423.5

EIF3C Polyclonal Antibody, Biotin Conjugated

A67133-050 50 ul
EUR 302.5

EIF3C Polyclonal Antibody, Biotin Conjugated

A67133-100 100 ul
EUR 423.5

EIF3C Polyclonal Antibody, HRP Conjugated

A67131
  • EUR 684.66
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 50 ul
  • 100 ul

EIF3C Polyclonal Antibody, FITC Conjugated

A67132
  • EUR 684.66
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 50 ul
  • 100 ul

EIF3C Polyclonal Antibody, Biotin Conjugated

A67133
  • EUR 684.66
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 50 ul
  • 100 ul

EIF3C sgRNA CRISPR Lentivector set (Human)

K0667201 3 x 1.0 ug
EUR 406.8

Eif3c sgRNA CRISPR Lentivector set (Rat)

K6287401 3 x 1.0 ug
EUR 406.8

Eif3c sgRNA CRISPR Lentivector set (Mouse)

K3973701 3 x 1.0 ug
EUR 406.8

EIF3C sgRNA CRISPR Lentivector (Human) (Target 1)

K0667202 1.0 ug DNA
EUR 184.8

EIF3C sgRNA CRISPR Lentivector (Human) (Target 2)

K0667203 1.0 ug DNA
EUR 184.8

EIF3C sgRNA CRISPR Lentivector (Human) (Target 3)

K0667204 1.0 ug DNA
EUR 184.8

Eif3c sgRNA CRISPR Lentivector (Rat) (Target 1)

K6287402 1.0 ug DNA
EUR 184.8

Eif3c sgRNA CRISPR Lentivector (Rat) (Target 2)

K6287403 1.0 ug DNA
EUR 184.8

Eif3c sgRNA CRISPR Lentivector (Rat) (Target 3)

K6287404 1.0 ug DNA
EUR 184.8

Eif3c sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3973702 1.0 ug DNA
EUR 184.8

Eif3c sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3973703 1.0 ug DNA
EUR 184.8

Eif3c sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3973704 1.0 ug DNA
EUR 184.8

EIF3C Protein Vector (Human) (pPB-C-His)

PV013861 500 ng
EUR 394.8

EIF3C Protein Vector (Human) (pPB-N-His)

PV013862 500 ng
EUR 394.8

EIF3C Protein Vector (Human) (pPM-C-HA)

PV013863 500 ng
EUR 394.8

EIF3C Protein Vector (Human) (pPM-C-His)

PV013864 500 ng
EUR 394.8

EIF3C Protein Vector (Mouse) (pPB-C-His)

PV175034 500 ng
EUR 1278

EIF3C Protein Vector (Mouse) (pPB-N-His)

PV175035 500 ng
EUR 1278

EIF3C Protein Vector (Mouse) (pPM-C-HA)

PV175036 500 ng
EUR 1278

EIF3C Protein Vector (Mouse) (pPM-C-His)

PV175037 500 ng
EUR 1278

EIF3C 3'UTR Luciferase Stable Cell Line

TU006730 1.0 ml
EUR 2799.6

Eif3c 3'UTR GFP Stable Cell Line

TU253882 1.0 ml Ask for price

Eif3c 3'UTR Luciferase Stable Cell Line

TU105715 1.0 ml Ask for price

EIF3C 3'UTR GFP Stable Cell Line

TU056730 1.0 ml
EUR 2799.6

Eif3c 3'UTR Luciferase Stable Cell Line

TU203882 1.0 ml Ask for price

Eif3c 3'UTR GFP Stable Cell Line

TU155715 1.0 ml Ask for price

EIF3C Protein Vector (Rat) (pPB-C-His)

PV265798 500 ng
EUR 1399.2

EIF3C Protein Vector (Rat) (pPB-N-His)

PV265799 500 ng
EUR 1399.2

EIF3C Protein Vector (Rat) (pPM-C-HA)

PV265800 500 ng
EUR 1399.2

EIF3C Protein Vector (Rat) (pPM-C-His)

PV265801 500 ng
EUR 1399.2

EIF3C Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV687175 1.0 ug DNA
EUR 1626

EIF3C Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV687179 1.0 ug DNA
EUR 1626

EIF3C Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV687180 1.0 ug DNA
EUR 1626

Eukaryotic Translation Initiation Factor 3 Subunit C (eIF3C) Antibody

20-abx141939
  • EUR 444.00
  • EUR 727.20
  • EUR 360.00
  • 100 ul
  • 200 ul
  • 50 ul

Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody

20-abx005318
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody

20-abx112371
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody

abx028765-400ul 400 ul
EUR 627.6

Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody

abx028765-80l 80 µl
EUR 343.2

Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody

20-abx317991
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody

abx232703-100ug 100 ug
EUR 610.8

Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody (HRP)

20-abx310516
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody (FITC)

20-abx310517
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody (Biotin)

20-abx310518
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

EIF3C sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K0667205 3 x 1.0 ug
EUR 451.2

Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K6287405 3 x 1.0 ug
EUR 451.2

Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K3973705 3 x 1.0 ug
EUR 451.2

Human Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) ELISA Kit

abx387094-96tests 96 tests
EUR 1093.2

EIF3C Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV687176 1.0 ug DNA
EUR 1626

EIF3C Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV687177 1.0 ug DNA
EUR 1695.6

EIF3C Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV687178 1.0 ug DNA
EUR 1695.6

EIF3C sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K0667206 1.0 ug DNA
EUR 200.4

EIF3C sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K0667207 1.0 ug DNA
EUR 200.4

EIF3C sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K0667208 1.0 ug DNA
EUR 200.4

Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K6287406 1.0 ug DNA
EUR 200.4

Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K6287407 1.0 ug DNA
EUR 200.4

Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K6287408 1.0 ug DNA
EUR 200.4

Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3973706 1.0 ug DNA
EUR 200.4

Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K3973707 1.0 ug DNA
EUR 200.4

Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K3973708 1.0 ug DNA
EUR 200.4

We describe the underlying ideas, summarize the developmental historical past and state-of-the-art, together with newest purposes in high-resolution microscopy, live-cell and single-molecule imaging. We additional talk about remaining limitations, that are (i) decrease photostabilization of most self-healing dyes when in comparison with answer components, (ii) restricted mechanistic understanding on the affect of the biochemical setting and molecular oxygen on self-healing, and (iii) the dearth of low cost and facile bioconjugation methods.

Lastly, we offer concepts on learn how to additional advance self-healing dyes, present new information on redox blinking attributable to double-stranded DNA and spotlight forthcoming work on intramolecular photostabilization of fluorescent proteins.