Enzymatic and genetic characterization of lignin depolymerization by Streptomyces sp. S6 isolated from a tropical environment.
The conversion of lignocellulosic biomass into bioethanol or biochemical merchandise requires an important pretreatment course of to breakdown the recalcitrant lignin construction. This analysis focuses on the isolation and characterization of a lignin-degrading bacterial pressure from a decaying oil palm empty fruit bunch (OPEFB).
The remoted pressure, recognized as Streptomyces sp. S6, grew in a minimal medium with Kraft lignin (KL) as the only carbon supply. A number of identified ligninolytic enzyme assays have been carried out, and lignin peroxidase (LiP), laccase (Lac), dye-decolorizing peroxidase (DyP) and aryl-alcohol oxidase (AAO) actions have been detected. A 55.3% discount within the molecular weight (Mw) of KL was noticed after 7 days of incubation with Streptomyces sp.
S6 primarily based on gel-permeation chromatography (GPC). Gasoline chromatography-mass spectrometry (GC-MS) additionally efficiently highlighted the manufacturing of lignin-derived fragrant compounds, reminiscent of 3-methyl-butanoic acid, guaiacol derivatives, and 4,6-dimethyl-dodecane, after therapy of KL with pressure S6. Lastly, draft genome evaluation of Streptomyces sp.
S6 additionally revealed the presence of robust lignin degradation equipment and recognized numerous candidate genes chargeable for lignin depolymerization, in addition to for the mineralization of the decrease molecular weight compounds, confirming the lignin degradation functionality of the bacterial pressure.
Self-Healing Dyes – Keeping The Promise?
Self-healing dyes have emerged as a brand new promising class of fluorescent labels. They include two items, a fluorescent dye and a photostabilizer. The latter heals at any time when the fluorescent dye is endangered of taking a response pathway in direction of photobleaching.
EIF3C Antibody |
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47321-100ul | SAB | 100ul | EUR 302.4 |
EIF3C siRNA |
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20-abx901680 | Abbexa |
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EIF3C siRNA |
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20-abx915165 | Abbexa |
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EIF3C siRNA |
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20-abx915166 | Abbexa |
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EIF3C Antibody |
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1-CSB-PA857867LA01HU | Cusabio |
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Description: A polyclonal antibody against EIF3C. Recognizes EIF3C from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:2000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
EIF3C Antibody |
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1-CSB-PA007531GA01HU | Cusabio |
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Description: A polyclonal antibody against EIF3C. Recognizes EIF3C from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
EIF3C Rabbit pAb |
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A7022-100ul | Abclonal | 100 ul | EUR 369.6 |
EIF3C Rabbit pAb |
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A7022-200ul | Abclonal | 200 ul | EUR 550.8 |
EIF3C Rabbit pAb |
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A7022-20ul | Abclonal | 20 ul | EUR 219.6 |
EIF3C Rabbit pAb |
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A7022-50ul | Abclonal | 50 ul | EUR 267.6 |
EIF3C Conjugated Antibody |
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C47321 | SAB | 100ul | EUR 476.4 |
EIF3C cloning plasmid |
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CSB-CL857867HU-10ug | Cusabio | 10ug | EUR 669.6 |
Description: A cloning plasmid for the EIF3C gene. |
Anti-EIF3C antibody |
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PAab02703 | Lifescience Market | 100 ug | EUR 463.2 |
anti- EIF3C antibody |
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FNab02703 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against EIF3C |
Anti-EIF3C antibody |
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STJ29102 | St John's Laboratory | 100 µl | EUR 332.4 |
pCMV-SPORT6-EIF3C |
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PVT14144 | Lifescience Market | 2 ug | EUR 594 |
EIF3C Polyclonal Antibody |
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A72797-050 | EpiGentek | 50 ul | EUR 302.5 |
EIF3C Polyclonal Antibody |
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A72797-100 | EpiGentek | 100 ul | EUR 423.5 |
EIF3C Polyclonal Antibody |
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A67130-020 | EpiGentek | 20 ul | EUR 117.7 |
EIF3C Polyclonal Antibody |
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A67130-050 | EpiGentek | 50 ul | EUR 302.5 |
EIF3C Polyclonal Antibody |
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A67130-100 | EpiGentek | 100 ul | EUR 423.5 |
EIF3C Polyclonal Antibody |
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A67130 | EpiGentek |
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EIF3C Polyclonal Antibody |
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A72797 | EpiGentek |
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EIF3C Antibody, HRP conjugated |
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1-CSB-PA857867LB01HU | Cusabio |
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Description: A polyclonal antibody against EIF3C. Recognizes EIF3C from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
EIF3C Antibody, FITC conjugated |
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1-CSB-PA857867LC01HU | Cusabio |
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Description: A polyclonal antibody against EIF3C. Recognizes EIF3C from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
EIF3C Antibody, Biotin conjugated |
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1-CSB-PA857867LD01HU | Cusabio |
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Description: A polyclonal antibody against EIF3C. Recognizes EIF3C from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
Human EIF3C shRNA Plasmid |
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20-abx955698 | Abbexa |
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Mouse EIF3C shRNA Plasmid |
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20-abx974708 | Abbexa |
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Rat EIF3C shRNA Plasmid |
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20-abx988626 | Abbexa |
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EIF3C ELISA KIT|Human |
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EF009339 | Lifescience Market | 96 Tests | EUR 826.8 |
Bovine EIF3C ELISA KIT |
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ELI-26098b | Lifescience Market | 96 Tests | EUR 1113.6 |
Mouse Eif3c ELISA KIT |
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ELI-09164m | Lifescience Market | 96 Tests | EUR 1038 |
Human EIF3C ELISA KIT |
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ELI-09999h | Lifescience Market | 96 Tests | EUR 988.8 |
Eif3c ORF Vector (Rat) (pORF) |
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ORF066450 | ABM | 1.0 ug DNA | EUR 607.2 |
EIF3C ORF Vector (Human) (pORF) |
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ORF003466 | ABM | 1.0 ug DNA | EUR 114 |
Eif3c ORF Vector (Mouse) (pORF) |
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ORF043759 | ABM | 1.0 ug DNA | EUR 607.2 |
EIF3C Polyclonal Antibody, HRP Conjugated |
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A67131-050 | EpiGentek | 50 ul | EUR 302.5 |
EIF3C Polyclonal Antibody, HRP Conjugated |
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A67131-100 | EpiGentek | 100 ul | EUR 423.5 |
EIF3C Polyclonal Antibody, FITC Conjugated |
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A67132-050 | EpiGentek | 50 ul | EUR 302.5 |
EIF3C Polyclonal Antibody, FITC Conjugated |
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A67132-100 | EpiGentek | 100 ul | EUR 423.5 |
EIF3C Polyclonal Antibody, Biotin Conjugated |
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A67133-050 | EpiGentek | 50 ul | EUR 302.5 |
EIF3C Polyclonal Antibody, Biotin Conjugated |
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A67133-100 | EpiGentek | 100 ul | EUR 423.5 |
EIF3C Polyclonal Antibody, HRP Conjugated |
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A67131 | EpiGentek |
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EIF3C Polyclonal Antibody, FITC Conjugated |
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A67132 | EpiGentek |
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EIF3C Polyclonal Antibody, Biotin Conjugated |
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A67133 | EpiGentek |
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EIF3C sgRNA CRISPR Lentivector set (Human) |
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K0667201 | ABM | 3 x 1.0 ug | EUR 406.8 |
Eif3c sgRNA CRISPR Lentivector set (Rat) |
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K6287401 | ABM | 3 x 1.0 ug | EUR 406.8 |
Eif3c sgRNA CRISPR Lentivector set (Mouse) |
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K3973701 | ABM | 3 x 1.0 ug | EUR 406.8 |
EIF3C sgRNA CRISPR Lentivector (Human) (Target 1) |
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K0667202 | ABM | 1.0 ug DNA | EUR 184.8 |
EIF3C sgRNA CRISPR Lentivector (Human) (Target 2) |
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K0667203 | ABM | 1.0 ug DNA | EUR 184.8 |
EIF3C sgRNA CRISPR Lentivector (Human) (Target 3) |
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K0667204 | ABM | 1.0 ug DNA | EUR 184.8 |
Eif3c sgRNA CRISPR Lentivector (Rat) (Target 1) |
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K6287402 | ABM | 1.0 ug DNA | EUR 184.8 |
Eif3c sgRNA CRISPR Lentivector (Rat) (Target 2) |
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K6287403 | ABM | 1.0 ug DNA | EUR 184.8 |
Eif3c sgRNA CRISPR Lentivector (Rat) (Target 3) |
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K6287404 | ABM | 1.0 ug DNA | EUR 184.8 |
Eif3c sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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K3973702 | ABM | 1.0 ug DNA | EUR 184.8 |
Eif3c sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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K3973703 | ABM | 1.0 ug DNA | EUR 184.8 |
Eif3c sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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K3973704 | ABM | 1.0 ug DNA | EUR 184.8 |
EIF3C Protein Vector (Human) (pPB-C-His) |
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PV013861 | ABM | 500 ng | EUR 394.8 |
EIF3C Protein Vector (Human) (pPB-N-His) |
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PV013862 | ABM | 500 ng | EUR 394.8 |
EIF3C Protein Vector (Human) (pPM-C-HA) |
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PV013863 | ABM | 500 ng | EUR 394.8 |
EIF3C Protein Vector (Human) (pPM-C-His) |
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PV013864 | ABM | 500 ng | EUR 394.8 |
EIF3C Protein Vector (Mouse) (pPB-C-His) |
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PV175034 | ABM | 500 ng | EUR 1278 |
EIF3C Protein Vector (Mouse) (pPB-N-His) |
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PV175035 | ABM | 500 ng | EUR 1278 |
EIF3C Protein Vector (Mouse) (pPM-C-HA) |
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PV175036 | ABM | 500 ng | EUR 1278 |
EIF3C Protein Vector (Mouse) (pPM-C-His) |
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PV175037 | ABM | 500 ng | EUR 1278 |
EIF3C 3'UTR Luciferase Stable Cell Line |
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TU006730 | ABM | 1.0 ml | EUR 2799.6 |
Eif3c 3'UTR GFP Stable Cell Line |
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TU253882 | ABM | 1.0 ml | Ask for price |
Eif3c 3'UTR Luciferase Stable Cell Line |
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TU105715 | ABM | 1.0 ml | Ask for price |
EIF3C 3'UTR GFP Stable Cell Line |
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TU056730 | ABM | 1.0 ml | EUR 2799.6 |
Eif3c 3'UTR Luciferase Stable Cell Line |
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TU203882 | ABM | 1.0 ml | Ask for price |
Eif3c 3'UTR GFP Stable Cell Line |
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TU155715 | ABM | 1.0 ml | Ask for price |
EIF3C Protein Vector (Rat) (pPB-C-His) |
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PV265798 | ABM | 500 ng | EUR 1399.2 |
EIF3C Protein Vector (Rat) (pPB-N-His) |
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PV265799 | ABM | 500 ng | EUR 1399.2 |
EIF3C Protein Vector (Rat) (pPM-C-HA) |
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PV265800 | ABM | 500 ng | EUR 1399.2 |
EIF3C Protein Vector (Rat) (pPM-C-His) |
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PV265801 | ABM | 500 ng | EUR 1399.2 |
EIF3C Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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LV687175 | ABM | 1.0 ug DNA | EUR 1626 |
EIF3C Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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LV687179 | ABM | 1.0 ug DNA | EUR 1626 |
EIF3C Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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LV687180 | ABM | 1.0 ug DNA | EUR 1626 |
Eukaryotic Translation Initiation Factor 3 Subunit C (eIF3C) Antibody |
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20-abx141939 | Abbexa |
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Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody |
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20-abx005318 | Abbexa |
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Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody |
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20-abx112371 | Abbexa |
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Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody |
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abx028765-400ul | Abbexa | 400 ul | EUR 627.6 |
Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody |
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abx028765-80l | Abbexa | 80 µl | EUR 343.2 |
Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody |
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20-abx317991 | Abbexa |
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Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody |
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abx232703-100ug | Abbexa | 100 ug | EUR 610.8 |
Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody (HRP) |
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20-abx310516 | Abbexa |
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Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody (FITC) |
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20-abx310517 | Abbexa |
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Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) Antibody (Biotin) |
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20-abx310518 | Abbexa |
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EIF3C sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) |
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K0667205 | ABM | 3 x 1.0 ug | EUR 451.2 |
Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat) |
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K6287405 | ABM | 3 x 1.0 ug | EUR 451.2 |
Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse) |
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K3973705 | ABM | 3 x 1.0 ug | EUR 451.2 |
Human Eukaryotic Translation Initiation Factor 3 Subunit C (EIF3C) ELISA Kit |
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abx387094-96tests | Abbexa | 96 tests | EUR 1093.2 |
EIF3C Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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LV687176 | ABM | 1.0 ug DNA | EUR 1626 |
EIF3C Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro) |
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LV687177 | ABM | 1.0 ug DNA | EUR 1695.6 |
EIF3C Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro) |
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LV687178 | ABM | 1.0 ug DNA | EUR 1695.6 |
EIF3C sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1) |
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K0667206 | ABM | 1.0 ug DNA | EUR 200.4 |
EIF3C sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2) |
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K0667207 | ABM | 1.0 ug DNA | EUR 200.4 |
EIF3C sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) |
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K0667208 | ABM | 1.0 ug DNA | EUR 200.4 |
Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1) |
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K6287406 | ABM | 1.0 ug DNA | EUR 200.4 |
Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2) |
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K6287407 | ABM | 1.0 ug DNA | EUR 200.4 |
Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3) |
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K6287408 | ABM | 1.0 ug DNA | EUR 200.4 |
Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1) |
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K3973706 | ABM | 1.0 ug DNA | EUR 200.4 |
Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2) |
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K3973707 | ABM | 1.0 ug DNA | EUR 200.4 |
Eif3c sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3) |
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K3973708 | ABM | 1.0 ug DNA | EUR 200.4 |
We describe the underlying ideas, summarize the developmental historical past and state-of-the-art, together with newest purposes in high-resolution microscopy, live-cell and single-molecule imaging. We additional talk about remaining limitations, that are (i) decrease photostabilization of most self-healing dyes when in comparison with answer components, (ii) restricted mechanistic understanding on the affect of the biochemical setting and molecular oxygen on self-healing, and (iii) the dearth of low cost and facile bioconjugation methods.
Lastly, we offer concepts on learn how to additional advance self-healing dyes, present new information on redox blinking attributable to double-stranded DNA and spotlight forthcoming work on intramolecular photostabilization of fluorescent proteins.