Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments.

The retina is a fancy tissue that initiates and integrates the primary steps of imaginative and prescient. Dysfunction of retinal cells is a trademark of many blinding illnesses, and future therapies hinge on basic understandings about how totally different retinal cells operate usually. Gaining such data with biochemical strategies has confirmed troublesome as a result of contributions of specific cell varieties are diminished within the retinal cell milieu.

Dwell retinal imaging can present a view of quite a few organic processes on a subcellular stage, due to a rising variety of genetically encoded fluorescent biosensors. Nonetheless, this system has up to now been restricted to tadpoles and zebrafish larvae, the outermost retinal layers of remoted retinas, or decrease decision imaging of retinas in dwell animals. Right here we current a technique for producing dwell ex vivo retinal slices from grownup zebrafish for dwell imaging by way of confocal microscopy.

This preparation yields transverse slices with all retinal layers and most cell varieties seen for performing confocal imaging experiments utilizing perfusion. Transgenic zebrafish expressing fluorescent proteins or biosensors in particular retinal cell varieties or organelles are used to extract single-cell data from an intact retina. Moreover, retinal slices could be loaded with fluorescent indicator dyes, including to the strategy’s versatility.

This protocol was developed for imaging Ca2+ inside zebrafish cone photoreceptors, however with correct markers it may very well be tailored to measure Ca2+ or metabolites in Müller cells, bipolar and horizontal cells, microglia, amacrine cells, or retinal ganglion cells.

The retinal pigment epithelium is faraway from slices so this methodology will not be appropriate for finding out that cell sort. With apply, it’s potential to generate serial slices from one animal for a number of experiments. This adaptable approach supplies a strong software for answering many questions on retinal cell biology, Ca2+, and power homeostasis.

Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments.
Making ready Recent Retinal Slices from Grownup Zebrafish for Ex Vivo Imaging Experiments.

Passive membrane penetration by ZnO nanoparticles is pushed by the interaction of electrostatic and section boundary situations.

The internalization of nanoparticles via the organic membrane is of immense significance for biomedical purposes. A basic understanding of the lipid specificity and the function of the membrane biochemical and bodily forces at play in modulating penetration are missing.

The present understanding of nanoparticle-membrane interplay is drawn largely from computational research and lacks adequate experimental proof. Herein, utilizing confocal fluorescence imaging and potentiometric dye-based fluorimetry, we first investigated the interplay of ZnONP in each multi-component and particular person lipid membranes utilizing cell-like big unilamellar vesicles to dissect the lipid specificity; additionally, we investigated the adjustments in membrane order, anisotropy and hydrophobicity. ZnONP was discovered to work together with phosphatidylinositol and phosphatidylcholine head-group-containing lipids particularly.

eIF3A

MBS125630-005mL 0.05mL
EUR 255

eIF3A

MBS125630-01mL 0.1mL
EUR 345

eIF3A

MBS125630-02mL 0.2mL
EUR 545

eIF3A

MBS125630-5x02mL 5x0.2mL
EUR 2265

EIF3A siRNA

20-abx915160
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

EIF3A siRNA

20-abx915161
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

EIF3A siRNA

20-abx901678
  • Ask for price
  • Ask for price
  • 15 nmol
  • 30 nmol

EIF3A Antibody

45975-100ul 100ul
EUR 302.4

EIF3A Antibody

45975-50ul 50ul
EUR 224.4

EIF3A Antibody

DF9510 200ul
EUR 420

EIF3A Antibody

DF9510-100ul 100ul
EUR 280

EIF3A Antibody

DF9510-200ul 200ul
EUR 350

eIF3A Antibody

E90573 100μg
EUR 255
Description: Available in various conjugation types.

EIF3A Antibody

E314885 100ug/200ul
EUR 295
Description: Available in various conjugation types.

EIF3A Antibody

E19-9510-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

EIF3A Antibody

E19-9510-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

EIF3A Antibody

1-CSB-PA623903LA01HU
  • Ask for price
  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3A. Recognizes EIF3A from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200

EIF3A Antibody

ABD9510 100ug
EUR 325

EIF3A Antibody

GWB-MW347E 50ug Ask for price

EIF3A Antibody

MBS7052861-005mg 0.05mg
EUR 190

EIF3A Antibody

MBS7052861-01mg 0.1mg
EUR 270

EIF3A Antibody

MBS7052861-5x01mg 5x0.1mg
EUR 1205

eIF3A Antibody

MBS8505314-01mg 0.1mg
EUR 325

eIF3A Antibody

MBS8505314-01mLAF405L 0.1mL(AF405L)
EUR 565

eIF3A Antibody

MBS8505314-01mLAF405S 0.1mL(AF405S)
EUR 565

eIF3A Antibody

MBS8505314-01mLAF610 0.1mL(AF610)
EUR 565

eIF3A Antibody

MBS8505314-01mLAF635 0.1mL(AF635)
EUR 565

EIF3A Antibody

MBS8518521-005mg 0.05mg
EUR 235

EIF3A Antibody

MBS8518521-01mg 0.1mg
EUR 305

EIF3A Antibody

MBS8518521-01mLAF405M 0.1mL(AF405M)
EUR 465

EIF3A Antibody

MBS8518521-01mLAF546 0.1mL(AF546)
EUR 465

EIF3A Antibody

MBS8518521-01mLAF750 0.1mL(AF750)
EUR 465

EIF3A Antibody

MBS9609827-01mL 0.1mL
EUR 260

EIF3A Antibody

MBS9609827-02mL 0.2mL
EUR 305

EIF3A Antibody

MBS9609827-5x02mL 5x0.2mL
EUR 1220

EIF6 Antibody / EIF3A

R32150 100 ug
EUR 356.15
Description: EIF6 (Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al. (1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al. (2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al. (2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50% reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.

EIF3A siRNA (Rat)

MBS8223200-15nmol 15nmol
EUR 405

EIF3A siRNA (Rat)

MBS8223200-30nmol 30nmol
EUR 565

EIF3A siRNA (Rat)

MBS8223200-5x30nmol 5x30nmol
EUR 2450

EIF3A Rabbit pAb

A0573-100ul 100 ul
EUR 369.6

EIF3A Rabbit pAb

A0573-200ul 200 ul
EUR 550.8

EIF3A Rabbit pAb

A0573-20ul 20 ul
EUR 219.6

EIF3A Rabbit pAb

A0573-50ul 50 ul
EUR 267.6

EIF3A Rabbit pAb

E2500573 100ul
EUR 225
Description: Available in various conjugation types.

eIF3A Rabbit mAb

57134 100ul
EUR 339

eIF3A Rabbit mAb

E2R30208 100ul
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

EIF3A cDNA Clone

MBS7046785-001mgPlasmid02mLGlycerolStock 0.01mgPlasmid+0.2mLGlycerol-Stock
EUR 1620

EIF3A cDNA Clone

MBS7046785-5x001mgPlasmid5x02mLGlycerolStock 5x0.01mgPlasmid+5x0.2mLGlycerol-Stock
EUR 7255

EIF3A Rabbit pAb

MBS8544060-01mL 0.1mL
EUR 305

EIF3A Rabbit pAb

MBS8544060-01mLAF405L 0.1mL(AF405L)
EUR 565

EIF3A Rabbit pAb

MBS8544060-01mLAF405S 0.1mL(AF405S)
EUR 565

EIF3A Rabbit pAb

MBS8544060-01mLAF610 0.1mL(AF610)
EUR 565

EIF3A Rabbit pAb

MBS8544060-01mLAF635 0.1mL(AF635)
EUR 565

EIF3A Rabbit pAb

A0573 50μL
EUR 262.15

EIF3A siRNA (Mouse)

MBS8202340-15nmol 15nmol
EUR 405

EIF3A siRNA (Mouse)

MBS8202340-30nmol 30nmol
EUR 565

EIF3A siRNA (Mouse)

MBS8202340-5x30nmol 5x30nmol
EUR 2450

EIF3A siRNA (Human)

MBS8228485-15nmol 15nmol
EUR 405

EIF3A siRNA (Human)

MBS8228485-30nmol 30nmol
EUR 565

EIF3A siRNA (Human)

MBS8228485-5x30nmol 5x30nmol
EUR 2450

EIF3A cloning plasmid

CSB-CL623903HU-10ug 10ug
EUR 1954.8
Description: A cloning plasmid for the EIF3A gene.

EIF3A Blocking Peptide

DF9510-BP 1mg
EUR 234

EIF3A Blocking Peptide

MBS9628531-1mg 1mg
EUR 380

EIF3A Blocking Peptide

MBS9628531-5x1mg 5x1mg
EUR 1650

EIF3A Polyclonal Antibody

ABP58469-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of EIF3A from Human, Mouse, Rat. This EIF3A antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human EIF3A protein

EIF3A Polyclonal Antibody

ABP58469-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of EIF3A from Human, Mouse, Rat. This EIF3A antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human EIF3A protein

EIF3A Polyclonal Antibody

ABP58469-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of EIF3A from Human, Mouse, Rat. This EIF3A antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human EIF3A protein

EIF3A Polyclonal Antibody

E11-200710 100ug/100ul
EUR 225
Description: Available in various conjugation types.

EIF3A Conjugated Antibody

C45975 100ul
EUR 476.4

EIF3A Polyclonal Antibody

BT-AP08780-100ul 100ul Ask for price
Description: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.,mass spectrometry: PubMed:17322308,PTM:Phosphorylated. Phosphorylation is enhanced upon serum stimulation.,Belongs to the eIF-3 subunit A family.,Contains 1 PCI domain.,subunit:Interacts with EIF4G1 (By similarity). Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3L and EIF3K. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of FRAP1 and RAPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with KRT7 and PIWIL2.,

EIF3A Polyclonal Antibody

BT-AP08780-20ul 20ul Ask for price
Description: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.,mass spectrometry: PubMed:17322308,PTM:Phosphorylated. Phosphorylation is enhanced upon serum stimulation.,Belongs to the eIF-3 subunit A family.,Contains 1 PCI domain.,subunit:Interacts with EIF4G1 (By similarity). Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3L and EIF3K. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of FRAP1 and RAPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with KRT7 and PIWIL2.,

EIF3A Polyclonal Antibody

BT-AP08780-50ul 50ul Ask for price
Description: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.,mass spectrometry: PubMed:17322308,PTM:Phosphorylated. Phosphorylation is enhanced upon serum stimulation.,Belongs to the eIF-3 subunit A family.,Contains 1 PCI domain.,subunit:Interacts with EIF4G1 (By similarity). Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3L and EIF3K. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of FRAP1 and RAPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with KRT7 and PIWIL2.,

EIF3A Polyclonal Antibody

E44H08317 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

EIF3A Polyclonal Antibody

E-AB-62282-120uL 120uL
EUR 320
Description: Unconjugated

EIF3A Polyclonal Antibody

E-AB-62282-200uL 200uL
EUR 530
Description: Unconjugated

EIF3A Polyclonal Antibody

E-AB-62282-60uL 60uL
EUR 200
Description: Unconjugated

EIF3A Polyclonal Antibody

E-AB-62282-each each Ask for price
Description: Unconjugated

eIF3A Polyclonal Antibody

MBS9237669-01mL 0.1mL
EUR 415

eIF3A Polyclonal Antibody

MBS9237669-5x01mL 5x0.1mL
EUR 1841

EIF3A Polyclonal Antibody

MBS8530044-01mg 0.1mg
EUR 305

EIF3A Polyclonal Antibody

MBS8530044-01mLAF405L 0.1mL(AF405L)
EUR 465

EIF3A Polyclonal Antibody

MBS8530044-01mLAF405S 0.1mL(AF405S)
EUR 465

EIF3A Polyclonal Antibody

MBS8530044-01mLAF610 0.1mL(AF610)
EUR 465

EIF3A Polyclonal Antibody

MBS8530044-01mLAF635 0.1mL(AF635)
EUR 465

EIF3A Polyclonal Antibody

MBS2540827-006mL 0.06mL
EUR 190

EIF3A Polyclonal Antibody

MBS2540827-012mL 0.12mL
EUR 265

EIF3A Polyclonal Antibody

MBS2540827-02mL 0.2mL
EUR 415

EIF3A Polyclonal Antibody

MBS2540827-5x02mL 5x0.2mL
EUR 1835

EIF3A Conjugated Antibody

MBS9453927-01mLAF350 0.1mL(AF350)
EUR 480

EIF3A Conjugated Antibody

MBS9453927-01mLAF405 0.1mL(AF405)
EUR 480

EIF3A Conjugated Antibody

MBS9453927-01mLAF488 0.1mL(AF488)
EUR 480

EIF3A Conjugated Antibody

MBS9453927-01mLAF555 0.1mL(AF555)
EUR 480

EIF3A Conjugated Antibody

MBS9453927-01mLBiotin 0.1mL(Biotin)
EUR 480

EIF3A Polyclonal Antibody

RD80081A-120uL 120μL
EUR 360
Description: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis . The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

EIF3A Polyclonal Antibody

RD80081A-200uL 200μL
EUR 630
Description: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis . The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

EIF3A Polyclonal Antibody

RD80081A-60uL 60μL
EUR 204
Description: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis . The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

Rat Eif3a ELISA Kit

ELI-06048r 96tests
EUR 756

Rat EIF3A ELISA Kit

ERE0054 96Tests
EUR 625.2

Goat EIF3A ELISA Kit

EGTE0054 96Tests
EUR 625.2

Human EIF3A ELISA Kit

EHE0054 96Tests
EUR 625.2

Mouse Eif3a ELISA KIT

ELI-06046m 96tests
EUR 736

Human EIF3A ELISA KIT

ELI-06047h 96tests
EUR 696

Human EIF3A ELISA Kit

ELA-E1885h 96tests
EUR 696

Human EIF3A ELISA KIT

EF006084 96tests
EUR 566

Mouse EIF3A ELISA Kit

EME0054 96Tests
EUR 625.2

Bovine EIF3A ELISA Kit

EBE0054 96Tests
EUR 625.2

Canine EIF3A ELISA Kit

ECE0054 96Tests
EUR 625.2

Rabbit EIF3A ELISA Kit

ERTE0054 96Tests
EUR 625.2

Rat EIF3A shRNA Plasmid

20-abx988556
  • Ask for price
  • Ask for price
  • 150 µg
  • 300 µg

Porcine EIF3A ELISA Kit

EPE0054 96Tests
EUR 625.2

EIF3A Antibody (C-term)

MBS9205823-008mL 0.08mL
EUR 210

EIF3A Antibody (C-term)

MBS9205823-04mL 0.4mL
EUR 430

EIF3A Antibody (C-term)

MBS9205823-5x04mL 5x0.4mL
EUR 1910

pLKO.1-eIF3a-shRNA1

PVT20026 2ug
EUR 280

Anserini EIF3A ELISA Kit

EAE0054 96Tests
EUR 625.2

EIF3A (untagged)-Human eukaryotic translation initiation factor 3, subunit A (EIF3A)

SC117805 10 µg Ask for price

Mouse EIF3A shRNA Plasmid

20-abx970142
  • Ask for price
  • Ask for price
  • 150 µg
  • 300 µg

Human EIF3A shRNA Plasmid

20-abx955696
  • Ask for price
  • Ask for price
  • 150 µg
  • 300 µg

Human EIF3A Protein Lysate

MBS8410982-002mg 0.02mg
EUR 545

Human EIF3A Protein Lysate

MBS8410982-5x002mg 5x0.02mg
EUR 2225

EIF3A Peptide - middle region

MBS3247098-01mg 0.1mg
EUR 180

EIF3A Peptide - middle region

MBS3247098-5x01mg 5x0.1mg
EUR 730

Polyclonal Antibody to eIF3A

MBS669082-0025mg 0.025mg
EUR 175

Polyclonal Antibody to eIF3A

MBS669082-01mg 0.1mg
EUR 325

Polyclonal Antibody to eIF3A

MBS669082-5x01mg 5x0.1mg
EUR 1465

EIF3A Antibody - middle region

MBS3222427-01mL 0.1mL
EUR 455

EIF3A Antibody - middle region

MBS3222427-5x01mL 5x0.1mL
EUR 1995

EIF3A Antibody, HRP conjugated

1-CSB-PA623903LB01HU
  • Ask for price
  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3A. Recognizes EIF3A from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

EIF3A Antibody, HRP conjugated

MBS7052862-005mg 0.05mg
EUR 190

EIF3A Antibody, HRP conjugated

MBS7052862-01mg 0.1mg
EUR 270

EIF3A Antibody, HRP conjugated

MBS7052862-5x01mg 5x0.1mg
EUR 1205

EIF3A Antibody, FITC conjugated

1-CSB-PA623903LC01HU
  • Ask for price
  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3A. Recognizes EIF3A from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

OACD03196-1ML - EIF3A Antibody

OACD03196-1ML 1ml
EUR 659

EIF3A Antibody, FITC conjugated

MBS7052863-005mg 0.05mg
EUR 190

EIF3A Antibody, FITC conjugated

MBS7052863-01mg 0.1mg
EUR 270

EIF3A Antibody, FITC conjugated

MBS7052863-5x01mg 5x0.1mg
EUR 1205

OAAN00111-50UL - EIF3A Antibody

OAAN00111-50UL 50ul
EUR 149

EIF3A Rabbit Polyclonal Antibody

53053 100ul
EUR 439

EIF3A Antibody, Biotin conjugated

1-CSB-PA623903LD01HU
  • Ask for price
  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against EIF3A. Recognizes EIF3A from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

EIF3A Rabbit Polyclonal Antibody

ES9656-100ul 100ul
EUR 124
Description: A Rabbit Polyclonal antibody against EIF3A from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

EIF3A Rabbit Polyclonal Antibody

ES9656-50ul 50ul
EUR 74
Description: A Rabbit Polyclonal antibody against EIF3A from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

OACD03196-100UL - EIF3A Antibody

OACD03196-100UL 100ul
EUR 189

OAAN00111-100UL - EIF3A Antibody

OAAN00111-100UL 100ul
EUR 249

OAAN00111-200UL - EIF3A Antibody

OAAN00111-200UL 200ul
EUR 389

EIF3A Antibody, Biotin conjugated

MBS7052864-005mg 0.05mg
EUR 190

EIF3A Antibody, Biotin conjugated

MBS7052864-01mg 0.1mg
EUR 270

EIF3A Antibody, Biotin conjugated

MBS7052864-5x01mg 5x0.1mg
EUR 1205

EIF3A Rabbit Polyclonal Antibody

MBS9464329-005mL 0.05mL
EUR 300

EIF3A Rabbit Polyclonal Antibody

MBS9464329-01mL 0.1mL
EUR 390

EIF3A Rabbit Polyclonal Antibody

MBS9464329-5x01mL 5x0.1mL
EUR 1610

Eif3a (untagged) - Mouse eukaryotic translation initiation factor 3, subunit A (Eif3a), (10ug)

MC224280 10 µg Ask for price

EIF3A (GFP-tagged) - Human eukaryotic translation initiation factor 3, subunit A (EIF3A)

RG221766 10 µg Ask for price

ARP84538_P050 - EIF3A Antibody (ARP84538_P050)

ARP84538_P050 100ul
EUR 389

Eif3a ORF Vector (Rat) (pORF)

ORF066448 1.0 ug DNA
EUR 2496

EIF3A (NM_003750) Human Over-expression Lysate

LS008661 100ug
EUR 960
Description: Transient overexpression lysate of eukaryotic translation initiation factor 3, subunit A (EIF3A)

Guinea Pig EIF3A ELISA Kit

EGE0054 96Tests
EUR 625.2

EIF3A ORF Vector (Human) (pORF)

ORF012910 1.0 ug DNA
EUR 424.8

Eif3a ORF Vector (Mouse) (pORF)

ORF043757 1.0 ug DNA
EUR 1886.4

Eif3a (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 3, subunit A (Eif3a)

MR222604 10 µg Ask for price

Eif3a (GFP-tagged) - Mouse eukaryotic translation initiation factor 3 subunit A (Eif3a), (10ug)

MG222604 10 µg Ask for price

EIF3A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit A (EIF3A)

RC221766 10 µg Ask for price

Human EIF3A Protein Lysate 20ug

IHUEIF3APLLY20UG each
EUR 361
Description: Human EIF3A Protein Lysate 20ug

EIF3A ELISA Kit (Mouse) (OKEI00448)

OKEI00448 96 Wells
EUR 920.4
Description: Description of target: RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 9.375 pg/mL

EIF3A ELISA Kit (Human) (OKEH01347)

OKEH01347 96 Wells
EUR 794.4
Description: Description of target: ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.23 ng/mL

EIF3A Peptide - C-terminal region

MBS3246426-01mg 0.1mg
EUR 180

EIF3A Peptide - C-terminal region

MBS3246426-5x01mg 5x0.1mg
EUR 730

EIF3A Peptide - C-terminal region

MBS3247097-01mg 0.1mg
EUR 180

EIF3A Peptide - C-terminal region

MBS3247097-5x01mg 5x0.1mg
EUR 730

EIF3A Peptide - C-terminal region

MBS3240212-01mg 0.1mg
EUR 180

EIF3A Peptide - C-terminal region

MBS3240212-5x01mg 5x0.1mg
EUR 730

EIF3A Antibody - C-terminal region

MBS3221699-01mL 0.1mL
EUR 455

EIF3A Antibody - C-terminal region

MBS3221699-5x01mL 5x0.1mL
EUR 1995

EIF3A antibody - C-terminal region

MBS3215309-01mL 0.1mL
EUR 455

EIF3A antibody - C-terminal region

MBS3215309-5x01mL 5x0.1mL
EUR 1995

EIF3A Antibody - C-terminal region

MBS3222426-01mL 0.1mL
EUR 455

EIF3A Antibody - C-terminal region

MBS3222426-5x01mL 5x0.1mL
EUR 1995

OCOA01120-20UG - EIF3A Protein Lysate

OCOA01120-20UG 20ug
EUR 269

EIF3A Over-expression Lysate Product

GWB-DC20D1 0.1 mg Ask for price

OPCD02884-10UG - EIF3A Recombinant Protein

OPCD02884-10UG 10ug
EUR 139

OPCD02884-50UG - EIF3A Recombinant Protein

OPCD02884-50UG 50ug
EUR 339

OPCD02884-200UG - EIF3A Recombinant Protein

OPCD02884-200UG 200ug
EUR 669

OKEH01347-96W - EIF3A ELISA Kit (Human)

OKEH01347-96W 96Wells
EUR 525

Eif3a sgRNA CRISPR Lentivector set (Rat)

K6605701 3 x 1.0 ug
EUR 406.8

EIF3A sgRNA CRISPR Lentivector set (Human)

K0667001 3 x 1.0 ug
EUR 406.8

Eif3a sgRNA CRISPR Lentivector set (Mouse)

K3834701 3 x 1.0 ug
EUR 406.8

EIF3A Antibody (C-term) Blocking peptide

MBS9219536-INQUIRE INQUIRE Ask for price

Lenti-ORF clone of EIF3A (mGFP-tagged)-Human eukaryotic translation initiation factor 3, subunit A (EIF3A)

RC221766L2 10 µg Ask for price

Lenti-ORF clone of EIF3A (mGFP-tagged)-Human eukaryotic translation initiation factor 3, subunit A (EIF3A)

RC221766L4 10 µg Ask for price

EIF3A 3'UTR GFP Stable Cell Line

TU056728 1.0 ml
EUR 1672.8

Eif3a 3'UTR GFP Stable Cell Line

TU253880 1.0 ml Ask for price

We additional investigated the interplay of ZnONP with three physiologically related membrane situations various in composition and dipole potential. We discovered that ZnONP interplay results in a photoinduced enhancement of the partial-to-complete section separation relying upon the membrane composition and ldl cholesterol content material.

Curiously, whereas the lipid order of a partially-phase-separated membrane remained unchanged upon ZnONP crowding, a fully-phase-separated membrane confirmed a rise within the lipid order. Strikingly, ZnONP crowding induced a contrasting impact on the fluorescence anisotropy of the membrane upon binding to the 2 membrane situations, in step with the measured diffusion coefficient.

ZnONP appears to preferentially penetrate via the liquid disordered areas of the membrane and the boundaries of the phase-separated areas pushed by the interaction between the electrostatics and section boundary situations, that are collectively dictated by the composition and ZnONP-induced lipid reorganization. The outcomes could result in a larger understanding of the interaction of membrane parameters and ZnONP interplay in driving passive penetration.